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Stool from HIV patient for modified Ziehl-Neelsen stain For more details, follow website @ http://universe84a.com/ Lab Diagnosis of Cyclospora cayetanensis Due to being smaller in size, intracellular habitat, and inability to properly take up many histological stains and therefore, diagnosis of C. cayetanensis can be very difficult. Methods of detection- Microscopic detection in stool samples of oocysts; recovering oocysts in intestinal fluid or small bowel biopsy specimens Detection of genes of oocyst sporulation and amplification by polymerase chain reaction (PCR) of C. cayetanensis. phase contrast microscopy to check for the spherical oocysts modified acid-fast staining to check for variable staining from pale to red Note: Fecal exams to identify oocysts, centrifuging a sample of feces in a sucrose solution and then transferring a small amount to a slide was found to be remarkably effective. Since detection is so hard, one negative result should not discount the possibility of C. cayetanensis: tests involving fresh stool samples over the next few days should also be considered. Treatment of Cyclosporiasis The most effective drug for the treatment of the protozoan is a seven-day course of oral trimethoprim-sulfamethoxazole (TMP-SMX).
